abs against total p38 mapk  (Cell Signaling Technology Inc)

Journal: Journal of Lipid and Atherosclerosis

Article Title: Curcumin Attenuates Acrolein-induced COX-2 Expression and Prostaglandin Production in Human Umbilical Vein Endothelial Cells

doi: 10.12997/jla.2020.9.1.184

Figure Lengend Snippet: Cells were pre-treated with curcumin (10 and 25 µM) for 30 minutes prior to acrolein treatment (10 µM). After 30 minutes of incubation, the cell lysates (20 µg) were prepared and western blot analysis was performed with Abs against phosphorylated PKCδ, p38, and CREB or total PKCδ, p38, and CREB. Quantitative data were obtained using an imaging densitometer (ImageJ version 1.52a software, NIH, Bethesda, MD, USA). Data were analyzed by the Student's t -test. Data represent the mean±standard deviation of results from 3 independent experiments. PKC, protein kinase C; CREB, cAMP response element-binding protein; HUVEC, human umbilical vein endothelial cell; Ab, antibody; p, phosphorylated. * p <0.05 vs. control group.

Article Snippet: Abs against phospho-specific p38 MAPK, protein kinase C (PKC) δ, CREB, and Abs against total p38 MAPK, PKCδ, and CREB were purchased from Cell Signaling Technology Inc. (Beverly, MA, USA).

Techniques: Incubation, Western Blot, Imaging, Software, Standard Deviation, Binding Assay, Control

Journal: Acta Neuropathologica

Article Title: Soluble VCAM-1 impairs human brain endothelial barrier integrity via integrin α-4-transduced outside-in signalling

doi: 10.1007/s00401-015-1417-0

Figure Lengend Snippet: Soluble VCAM-1 activates Rho GTPase and p38 MAP kinase in brain endothelial cells. a Western blot analysis of Rho activation in integrin α-4-positive primary HBMEC after stimulation with 5 µg/mL sVCAM-1 for 10 min. b Western blot analysis of p38 MAP kinase activation after stimulation with 5 µg/mL sVCAM for the indicated durations. Stimulation with 12- O -tetradecanoylphorbol-13-acetate (TPA) and ionomycin for 10 min was used as a positive control. Representative of four independent experiments with different primary cell preparations

Article Snippet: After peroxidase inactivation, membranes were reprobed with Abs against total p38 (cat.-no. 9212, Cell Signaling), ERK (cat.-no. sc-94, Santa Cruz) or JNK (cat.-no. sc-474, Santa Cruz).

Techniques: Western Blot, Activation Assay, Positive Control

Journal: Acta Neuropathologica

Article Title: Soluble VCAM-1 impairs human brain endothelial barrier integrity via integrin α-4-transduced outside-in signalling

doi: 10.1007/s00401-015-1417-0

Figure Lengend Snippet: Effects of sVCAM-1 on tight junction morphology are partially mediated by Rho GTPase and p38 MAP kinase activation. Immunocytochemical analysis of F-actin ( a ) and occludin ( b ) expression in primary HBMEC which were left untreated, treated with 2 µM Rho-associated kinases inhibitor Y-27632 or 10 µM p38 inhibitor SB203580 for 2 h, 5 µg/mL sVCAM-1 for 1 h, or by one of the inhibitors for 2 h and additional presence of 5 µg/mL sVCAM-1 for the last hour. Representative of five independent experiments evaluated by blinded investigators. Scale bar 25 µm

Article Snippet: After peroxidase inactivation, membranes were reprobed with Abs against total p38 (cat.-no. 9212, Cell Signaling), ERK (cat.-no. sc-94, Santa Cruz) or JNK (cat.-no. sc-474, Santa Cruz).

Techniques: Activation Assay, Expressing